Web1 Mar 2014 · The site-specific coupling was accomplished by mutation of single amino acids on GGBP to cysteine and subsequent thiol conjugation. The resulting SPR surfaces had glucose-specific binding ... WebProduct Support. Reagent for performing ligand and/or surface thiol couplings. Reactive disulfide groups are introduced onto carboxyl groups of either the sensor chip matrix or …

Ligand immobilization using thiol-disulphide exchange

WebSPR can occur when plane-polarized light hits a metal film under total internal reflection conditions. SPR signal is directly dependent on the refractive index of the medium on the … Web22 Nov 2024 · The Biacore T200 is an instrument for Surface Plasmon Resonance (SPR), an optical technique that measures changes in refractive index near a metal surface over … buttered ritz crumbs

A surface acoustic wave (SAW)-enhanced grating-coupling phase ...

Web25 Sep 2015 · This is traditionally known as thiol coupling or thiophilic adsorption [28, 29]. In contrast, disulfide bond formation between SAVSBPM32 and BLA-L-SBP(A18C) in this study is expected to be guided or enhanced by the specific binding interactions between streptavidin and SBP. The efficiency for disulfide bond formation should be much higher … WebWe have proven that pyridine–boryl complexes can be used as superelectron donors to promote the coupling of thiols and aromatic halides through a S RN 1 mechanism. The … WebSPR-based kinetic affinity measurements are performed using a Biacore T200 instrument capable of automated or manual surface preparation, fully-automated injection of test samples ... chemistry is typical, though thiol and other coupling chemistries are also available. This method produces a stable biosensor surface that can be used for many … cd rates clark county wa