Phospho-yap1
WebJun 19, 2024 · The transcriptional coactivator YAP1 (yes-associated protein 1) regulates cell proliferation, cell-cell interactions, organ size, and tumorigenesis. ... genetic experiments revealed that intact androgen receptor signaling is necessary for androgen's inactivating effect on phospho-Ser-127 levels and increased YAP1 nuclear entry. We also found ... WebMonoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH.
Phospho-yap1
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WebWestern blot - Phospho-YAP1-S127 Rabbit pAb (AP0489) Western blot analysis of NIH/3T3, using Phospho-YAP1-S127 antibody (AP0489) at 1:400 dilution.NIH/3T3 cells were … WebBackground Information. The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their …
WebIn line with this findings there was an increased level of non-phospho-(Active)YAP1(Ser127) and non-phospho-(Active)β-catenin(Ser33/37/Thr41) in the lung tissue (Fig. 3 B-C). In … WebWB analysis using 29018-1-AP. Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29018-1-AP (Phospho-YAP1 (Ser397) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH …
WebAnti-phospho-YAP1 (pTyr357) antibody produced in rabbit Synonym (s): Anti-YAP, Anti-YAP2, Anti-YAP65, Anti-Yes-associated protein 1, 65kDa Match Criteria: Product Name, Keyword All Photos ( 1) Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal Match Criteria: Product Name, Keyword All Photos ( 25) WebAll lanes : Anti-YAP1 (phospho Y357) antibody [EPR23680-48] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate Lane 2 : HeLa whole cell lysate (phosphatase treated membrane) Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution ...
WebPhosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell …
WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein. Background YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. popular songs that came out in 2022WebFeb 27, 2024 · A phospho-resistant form of Yap1 was relocalized to the nucleus when Fat4 was downregulated , further supporting the conclusion that the nuclear translocation of Yap1 downstream of Fat4 is not ... popular songs that don\u0027t swearpopular songs to play on keyboardWebImmunocytochemistry analysis of Phospho-YAP1 (Ser127) in HeLa. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and … popular songs that came out in 2005WebPhospho-YAP1 is retained in the cytoplasm. In the absence of Hippo signaling, YAP1 translocates to the nucleus, associates with co-activators TEAD1-4, and functions as a transcriptional factor promoting the expression of key target genes. Components of the Hippo pathway are mutated in human cancers, and deregulation of this pathway plays a … popular songs to play on the guitarWebAnti-phospho-YAP1 (pTyr357) antibody produced in rabbit has been used in western blotting [ 2] and immunofluorescence. Packaging 25, 100, 200 μL Biochem/physiol … sharks cell phone lightsWebYAP1 Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein … popular songs that start with g