WebIonizing radiation (IR)–induced DNA damage results in the formation of IR-induced foci (iRIF) in the nucleus. The iRIF formation is part of the DNA damage response (DDR), … WebMar 7, 2024 · The siRNA-mediated UBA1 knockdown impaired the formation of both ubiquitin conjugates at the sites of DNA damage and IR-induced foci (IRIF) by the downstream components of the DNA double-strand breaks (DSBs) response pathway, 53BP1 and BRCA1.
SPOC1 modulates DNA repair by regulating key determinants of
WebAug 1, 2012 · Additionally, studies have shown that radiation induced foci (RIF), which are larger and distinct than normal IRIF, can arise after radiation exposure, that they can persist for long periods and do not always represent DSBs [38]. Their origin is currently unclear … In the case of a DNA damage-induced ssDNA gap two clamps would be placed … One recent study circumvented the foci background issue by looking at live cells … DNA ligase IV functions in DNA nonhomologous end-joining and V(D)J … Introduction. X-rays have been a source of therapy, as well as untoward biologic … Environmental DNA damage can be produced by physical or chemical … 1.. IntroductionNijmegen breakage syndrome (NBS) is a rare autosomal … To monitor IR-induced DSB repair, the induction and rate of loss of γH2AX foci … Table 1. Summary of the data measuring the numbers of γ-H2AX foci per cell in … Prevailing evidence suggests that the Mre11 complex functions in an ATM … Webmay be induced by exogenous DNA damaging agents. The cell utilizes two principal pathways for the repair of DSBs: homol-ogous recombination (HR) and nonhomologous recombina-tion (NHR) (29, 62). Homology-mediated repair requires an undamaged template molecule that contains a homologous DNA sequence ordinarily on a sister chromatid or a … ctpms.rg-pharma.com
Ionizing radiation-induced foci persistence screen to discover ...
WebAug 13, 2004 · The IR-induced intra-S-phase checkpoint reduces RDS. Compared with control cells, Mcph1-inhibited cells showed higher, but variable, RDS when exposed to 5- or 10-Gy irradiation (data not shown), and significantly higher RDS when irradiated with 20 Gy ( Fig. 4A ). This result indicates a role of Mcph1 in the intra-S-phase checkpoint. WebJul 28, 2010 · The dynamics of these IR-induced foci (IRIF) have been implicated in DNA damage signaling and DNA repair. As an IRIF reporter, we tracked the relocalization of green fluorescent protein fused to a chromatin binding domain of the checkpoint adapter protein 53BP1 after IR of breast cancer cells and tumors. WebIonizing radiation-induced foci persistence screen to discover enhancers of accelerated senescence Int J High Throughput Screen. 2011 Mar;2:1-13. doi: 10.2147/IJHTS.S17076. Authors Edwardine Labay 1 , Elena V Efimova 1 , Benjamin K Quarshie 1 , Daniel W Golden 1 , Ralph R Weichselbaum 1 , Stephen J Kron 1 Affiliation ct pmp sign in