Gm-130 antibody-directed staining

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August undefined, 2024

WebGOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane … WebIndependent Antibodies: Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex, colon, lymph node and testis using …

(PDF) Compensatory increase of VE-cadherin expression through …

WebImmunohistochemistry analysis of GM130 in paraffin-embedded human gastric cancer using GM130 Polyclonal Antibody (Product # PA5-95727) at a ... Cis- and trans-Golgi structure as visualized by GM130 and … WebFixation / permeabilization. Optimize protocol for best results with both antibodies. Separate testing may be required. Rinse. Dilute each antibody to its recommended dilution in antibody dilution buffer; incubate overnight at 4°C. Rinse. Perform any counterstaining steps using complementary reagents, mount in anti-fade reagent. The pros and ... honda dealer in north attleboro ma

GM130 Antibody SCBT - Santa Cruz Biotechnology

WebApr 6, 2024 · Immunogold staining was employed to detect the PI4P on sEVs. The results showed that the PI4P antibody labels were observed on the sEVs. The GFP labels of the GFP-tagged PI4P sensor were also observed on sEVs, while the secondary antibody negative control was not labelled (Figure 1g). The antibody cannot penetrate the cell … WebGM130 Polyclonal Antibody detects GM130 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GM130 protein stained by GM130 Polyclonal Antibody ( Product # PA5-85643) diluted at 1:200. Blue: Hoechst 33342 staining. WebII. Primary Antibody Incubation. Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product webpage) in 10 ml … honda dealer in new orleans la

GM-CSF Antibody, anti-human Hybridomas MACS Antibodies …

Alexa Fluor® 488 Mouse anti-GM130 - BD Biosciences

WebImmunofluorescent staining of human cell lines. HeLa cells (ATCC CCL-2) were cultured, fixed, permeabilized with Triton™ X-100, stained with Alexa Fluor® 488 Mouse anti-GM130 (pseudo colored green), and counter stained with Hoechst 33342 (pseudo colored blue) according to the Recommended Assay Procedure. WebAnti-GM130 (green) antibodies identified GM130-expressing and GM130-depleted cells. Nuclei were counterstained in blue. When compared with non-transfected cells visible on the same field, merged apotome views showed higher giantin staining in cells positive for FLAG tag (arrowheads), and lower giantin staining in cells negative for GM130 ... history in 80 year blocks honda dealer in murfreesboro tn

"WebNormal Function. Collapse Section. The BICD2 gene provides instructions for making one of a family of proteins called golgins. Golgins help maintain the structure of a cell … " - Gm-130 antibody-directed staining

Gm-130 antibody-directed staining

GOLGA2/GM130 antibody (CL488-11308) Proteintech

WebJan 10, 2024 · Datasheets. GM130 Antibody (B-10) is a mouse monoclonal IgG 2a κ, cited in 28 publications, provided at 200 µg/ml. raised against amino acids 191-255 mapping within an internal region of GM130 of … WebFeb 5, 2024 · Immunohistochemistry (IHC) is based upon the specific detection of an antigen, using an antibody to recognize it and then staining the resulting complex. This is why it is also called...

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WebThis highly specific GM130 antibody is suitable for use in WB, IHC-P and Direct ELISA with human, mouse and rat samples and is guaranteed to work as stated on the product data sheet. RQ4570 ... Immunofluorescent staining of FFPE human U-2 OS cells with GM130 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate ... WebIn biochemistry, immunostaining is any use of an antibody -based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue …

WebRabbit anti-β-catenin (C-2206) antibody, that the observed upregulation of VE-cadherin and ETS1 mouse anti-N-cadherin (C3865) and mouse anti-β-actin expression via NF-κB is part of the transcriptional pro- (A-5441) antibodies were obtained from Sigma-Aldrich. gram that endothelial cells implement to tune vascular Mouse anti-VE-cadherin (BV6 ... WebDec 5, 2024 · Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GM130 Antibody (H-7) has 22 citations in a variety of scientific publications. …

WebProteintech's Rabbit Polyclonal GOLGA2/GM130 antibody is validated in FC (Intra), IF and shows reactivity with human, Canine samples. ... IF Staining of HepG2 using CL647-11308. ... in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20247635) ... WebThe 35/GM130 monoclonal antibody recognizes GM130, regardless of phosphorylation status. Skip to ... BD Horizon™ Brilliant Stain Buffer. RY586 Reagent Promo. Lab Week 2024. View All Promotions. …

WebMay 12, 2024 · Antigen detection by dFA. The rabies antibody used for the dFA test is primarily directed against the nucleoprotein (antigen) of the virus (see The Virus section on viral structure). Rabies virus replicates in the …

WebDec 5, 2024 · GM130, a cis-Golgi matrix protein, interacts specifically with p115 and provides a membrane docking site. Both GM130 and p115 are involved in vesicle tethering to Golgi membranes. The amino-terminus of GM130 binds to p115, whereas the carboxy-terminus binds to Golgi membranes. Both Giantin and GM130 compete for binding to p115. honda dealer in north carolinaWebDec 27, 2016 · Histological analysis by Nissl staining revealed a dramatic loss of Purkinje cells in adult GM130-nKO cerebellum (Fig. 3C). By comparing the cerebellum at ... The … history in coins nottinghamWebMay 15, 2024 · Antibodies used at too high of a concentration can result in off-target staining.32–35 While determination of signal to noise ‘by eye’ is often used, to be … honda dealer in north aurora ilWebGolgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. history in 1939WebBoth methods involve exposure of fixed cells or tissues to primary antibodies directed against one or more proteins of interest. Bound antibodies are then detected using … history in german languageWebWhen antibodies bind to or block these targets they disrupt functioning, resulting in neurologic and psychiatric symptoms. 6. The autoantibodies may also trigger inflammation (encephalitis) in the basal ganglia. This … history in decemberWebGM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry. … honda dealer in orem ut service dept