WebApr 1, 2024 · In this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle amplification (RCA) for the detection of Cronobacter spp. in milk. The tailing single-stranded DNA was generated through AT-PCR and used to initiate RCA, generating tandem repetitive G … WebDive into the research topics of 'Dual asymmetric PCR: One-step construction of synthetic genes'. Together they form a unique fingerprint. Sort by Weight Alphabetically Medicine & Life Sciences. Synthetic Genes 100%. Polymerase Chain Reaction 42%. Oligonucleotides 37%. Chemical Compounds. Oligonucleotide 62% ...
Robust and scalable barcoding for massively parallel long-read
WebIn this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle … Cronobacter spp. are … WebMay 4, 2012 · Later, PCR technique was used in gene synthesis, and a series of new methods such as the dual asymmetric PCR and assemble PCR were developed , . To … land for sale paint rock texas
Visual single cell detection of dual-pathogens based on multiplex …
WebDownload scientific diagram (a) Agarose gel electrophoresis of asymmetric polymerase chain reaction (PCR) products obtained from 1 ng/µL of template DNA in the reaction mixture for five ... WebHere we describe a simple and rapid method for assembly and PCR-based accurate synthesis (PAS) of long DNA sequences. The PAS protocol involves the following five ... Advertisement. ... R.A. & Kline, B.C. Dual asymmetric PCR: one-step construction of synthetic genes. Biotechniques 12, 14–16 (1992). Young, L. & Dong, Q. Two-step total … WebJan 1, 1992 · We have developed a one-step process for constructing synthetic genes. Four adjacent oligonucleotides 17-100 bases in length having short overlaps of 15-17 bases … land for sale palm beach county fl