Ctll-2 ed50
WebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED 50 for this effect is 3 ng/mL. ... (Interleukin-2) protein, Human (60701GMP) stimulates the proliferation of CTLL-2 cells. The ED50 is 1.5 ng/mL. IL2 (Interleukin-2) protein, Human (60701GMP) was separated on SDS-PAGE under the reducing condition and visualized by Coomassie ... WebJun 2, 2012 · FIGURE 61.1. A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the …
Ctll-2 ed50
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Web1.875 x 10^4. HT-2. 2.151 x 10^4. The specific activity (units/mg) of a cytokine is typically calculated using a bioassay with one set of conditions and parameters, and a defined cell line. Due to inherent variability, the specific activity measured with a different cell line, cultivated under different conditions in a different lab, can differ ... WebSubculture Routine. Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate …
WebThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10^6 units/mg. ... natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also ... WebED50 is < 10 ng/mL as determined by the dose-dependant stimulation of the proliferation of CTLL-2 cell, corresponding to a specific activity of > 1 x 10 5 IU/mg. Recommend Usage: Biological Activity ... Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. ...
WebBioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL-2 cells. The ED50 is typically less than 1 ng/mL. The international units of CTGrade rh IL-15 is approximately 2.7 x 10. 5; IU/µg, which is calibrated against recombinant Human Interleukin 15 … WebBiological Activity: The ED50 as determined by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells is less than 10 ng/ml. Sequence ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRDPALV HQRPAPPSTV TTAGVTPQPE SLSPSGKEPA …
WebR&D Systems™ Recombinant Human IL-2 Protein. Version with mutation found in aldesleukin Available! Supplier: R&D Systems™ 202IL050. View more versions of this …
WebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa. city hall hull todayWebRecombinant Human Interleukin-2(IL2),Partial (Active) Supplier Page. Supplier Page from Biorbyt for Human IL2 protein. Product Specs; ... The ED50 as determined in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells is less than 0.5 ng/ml. city hall hull mapWebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific … did anyone win powerball april 9 2022WebBackground: Interleukin 2 (IL-2) is a T cell stimulatory cytokine best known for inducing activated T cell proliferation . in vitro ... Bioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade rh IL-2. city hall hull seating planWebED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: sequential chromatography ... IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus ... city hall humble txWebApr 4, 2024 · The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain γc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβ and γc. city hall human resources philadelphiaWebCorning ® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Twice per week. Some Important Considerations in Handling CTLL-2, … city hall huntington beach